Open positions
We are currently looking for people
to implement the NCN OPUS-17 and NCN SONATA-BIS project's:
OPUS-17: Short description of the project
Cell differentiation is a stepwise process whereby intrinsic and extrinsic factors modulate gene expression patterns to yield a cell of a particular identity. Transcriptional regulation is thus at the heart of this process. Distal DNA cis regulatory elements including enhancers impact the activity of the gene promoters thereby regulating the production of mRNA molecules. One of the essential questions is how enhancers find their target genes with precision. CTCF-bound insulators can shield promoters from impact of distal enhancers. Dysfunctions of insulators can lead to diseases including developmental syndromes and cancer.
PostDoc
We are looking for Postdoctoral Investigator!
Previous research and Your role
Previously, we have shown that differentiation of pluripotent stem cells is accompanied by a widespread gain of strength of CTCF-bound insulators (see paper). The mechanism underlying this phenomenon is unknown. Our preliminary data revealed a defined group of protein partners of CTCF that might orchestrate the overall gain of insulatory functions of CTCF in differentiation. We have obtained knockout lines for these factors. Your project will be to address the impact of these proteins on chromatin structure and activity by molecular biology approaches (ATAC-seq, Hi-C).
You have
Candidates should hold a PhD in molecular biology, biotechnology, or genetics. We require independence and excellent work organization skills, a creative approach to research problems, team collaboration skills and motivation are a must.
For more information about the position, please contact a.pekowska@nencki.edu.pl
SONATA BIS - 11: Short description of the project
ATP, the energy currency in the living world, is present in extremely high concentration in the cell. Remarkably, ATP can also act as a solvent able to disrupt protein aggregates. We have shown in the past that reduction of ATP in the cell leads to the emergence of chromatin loops connecting specific pairs of active regulatory elements including promoters and enhancers. In this interdisciplinary project, we will bridge high throughput genomics and genome editing technologies and computational tools to determine the role of ATP in regulating promoter-enhancer dialogue.
PostDoc
We are looking for Postdoctoral Investigator!
Your role
The postdoctoral investigator will take advantage of cutting-edge molecular and cell biology techniques, including ChIP-seq, RNA-seq, Hi-C, CRISPR-Cas9 and confocal microscopy to assess the impact of ATP on the interactions between cis-regulatory elements. The successful candidate will also have the opportunity to expand skills in the field of bioinformatics and quantitative biology.
You have
Candidates should hold a PhD in molecular biology or genetics. We require independence and excellent work organization skills, a creative approach to research problems, team collaboration skills and motivation. Experience in laboratory work is necessary.
For more information about the position, please contact a.pekowska@nencki.edu.pl
